| 摘要 |
The invention discloses a family of cloning vectors capable of cloning nucleic acid inserts of interest of long sizes, with low or reduced background and high efficiency of excision and method for preparing these vectors and library thereof.As example, it is disclosed a cloning vector comprising a construction vector segment (CS) and a replaceable segment (RS), wherein the size of CS is: 36.5 kb </= CS </= 38 kb, preferably CS is 37.5 kb, comprising lox recombination sites for Cre-recombination and/or att recombination sites for Gateway-like recombination, preferably also a background-reducing system selected from the group of: the ccdB gene, a lox sequence, the lacZ gene, and asymmetric site sequences recognized by restriction endonucleases.
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