FLUORESCENCE DETECTOR GEOMETRY

摘要

Excitation light (103) of a selected wavelength from an excitation monochromator is directed along the long axis of a flow cell (100) containing the sample to be analyzed, generating fluorescence. An emission monochromator is positioned at right angles to the plane of the excitation monochromator and receives the fluorescence from the flow cell (100) utilizing optical components positioned such that the entrance slit of the emission monochromator is aligned with the long axis of the emission window. The intensity of the output from the flow cell (100) is further maximised by positioning a retro-reflecting mirror (104) at the end of the flow channel (107) to effectively double the path-length of the excitation beam, and a reflecting surface on the side of the cell opposite the emission window to increase the collection efficiency and thereby increase the sensitivity of the detector.