<p>A method for balancing multiplexed PCR methods is provided. In the method, t wo or more sequential temporal PCR stages are used to effectively separate two or more PCR reactions in a single tube as an alternative to primer limiting to modulate the relative rate of production of a first amplicon by a first prim er set and a second amplicon by a second primer set during the first and second amplification stages. Also provided are rapid RT-PCR methods that find particular use in intraoperative diagnoses and prognoses, for instance in diagnosing malignant esophageal adenocarcenoma by determining expression levels of carcinoembryonic antigen (CEA) in sentinel lymph nodes.</p>
申请公布号
CA2439402(A1)
申请公布日期
2002.09.12
申请号
CA20022439402
申请日期
2002.03.04
申请人
UNIVERSITY OF PITTSBURGH OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION</SNM
发明人
KELLY, LORI A.;GODFREY, TONY E.;FINKELSTEIN, SYDNEY D.;RAJA, SIVA;LUKETICH, JAMES D.