NUCLEOTIDES AND POLYNUCLEOTIDES IMMOBILISED ON A SOLID SUPPORT VIA A DISULPHIDE LINKAGE

摘要

A method of immobilising a nucleotide or polynucleotide on a solid support, comprises: providing the solid support having a functionalised surface of the formula B - S - S - L where B is the solid support and L is a leaving group; providing a nucleotide or polynucleotide comprising at least one phosphorothioate group; and coupling the two together by means of a sulphide exchange reaction. The product has the structure B - S - S - P(O)R<1>R<2>, where - POR<1>R<2> represents a nucleotide or a polynucleotide. Arrays of the immobilised polynucleotides are useful for performing APEX or SBH assays.