USE OF FUSION PROTEINS WHOSE N-TERMINAL PART IS A HIRUDIN DERIVATIVE FOR THE PRODUCTION OF RECOMBINANT PROTEINS VIA SECRETION BY YEASTS

摘要

The invention relates to an expression cassette of the form P>x<-S>x<-B>n<-(ZR)-Hir (As>m<R)-protein (Y) - T, where P>x< is any promoter DNA sequence, selected in such a way that optimal yields of the protein of interest become achievable; S>x< is any DNA encoding a signal sequence or leader sequence which allows optimal yields; B>n< is 1-15 amino acid codons or a chemical bond; Z is the codon of a amino acid selected from the group comprising Lys and Arg; R is an Arg codon or a chemical bond; As>m< is a chemical bond or m amino aid codons, where m= 1-10; Hir is a DNA sequence coding for hirudin or a hirudin derivative which is at least 40 % homologous to natural hirudin; protein Y is a DNA sequence encoding any protein which can be produced in and secreted by yeast; T is an untraslated DNA sequence which is advantageous to the expression.