1,2-dioxetane derivates, luminescent reagents, luminescence methods and measuring methods

摘要

A 1,2-dioxetane derivative of the formula (I): wherein each of R<1> and R<2>, which are independent of each other, is a hydrogen atom, an alkyl group or an aryl group, or R<1> and R<2> may together form a cyclic or polycyclic organic ring group spiro-bonded to the dioxetane ring, R<3> is an alkyl group or an aryl group, or R<3> and R<1> or R<2> may together form a condensed ring containing the dioxetane ring and a hetero atom, and Ar is a group of the formula (A): wherein R<4> is a hydroxyl group, an alkoxyl group, an aralkyloxy group, a group of -OSi(R<5>R<6>R<7>) (wherein each of R<5>, R<6> and R<7>, which are independent of one another, is an alkyl group or an aryl group), a phosphate group or a group of -S(C=O)R<8> (wherein R<8> is an alkyl group or an aryl group), each of R<9> and R<10>, which are independent of each other, is a hydrogen atom, an alkyl group, an aryl group or a halogen atom, X is a halogen atom, and V is an oxygen atom or a sulfur atom, or a group of the formula (B): wherein R<4> is the same as R<4> in the above formula (A), each of R<11> and R<12>, which are independent of each other, is a hydrogen atom, an alkyl group, an aryl group or a halogen atom, Y is a halogen atom, and W is an oxygen atom or a sulfur atom.