发明名称 |
Method of synthesizing a fluorescently labeled protein |
摘要 |
The present invention provides a method of synthesizing a fluorescently labeled protein in a cell free protein synthesis system, comprising the steps of: (a) incubating a sample of ribosomes obtained from a cell-free extract with plasmid DNA containing a coding sequence for a protein of interest, said sample incubated in a coupled transcription/translation medium together with a aminoacyl tRNA having fluorescent label; (b) partially purifying said fluorescently labeled protein by separating newly synthesized, fluorescently-labeled protein from other fluorescent components within said sample; (c) measuring the amount of protein synthesized; (d) determining fluorescence of newly synthesized protein; and (e) determining the biological activity of the newly synthesized protein.
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申请公布号 |
US6448033(B1) |
申请公布日期 |
2002.09.10 |
申请号 |
US19960590729 |
申请日期 |
1996.01.24 |
申请人 |
RESEARCH DEVELOPMENT FOUNDATION |
发明人 |
KUDLICKI WIESLAW;KRAMER GISELA;HARDESTY BOYD |
分类号 |
C12N15/09;C07K1/107;C12N9/10;C12P21/02;G01N33/533;(IPC1-7):C12P21/06;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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