摘要 |
medicine. SUBSTANCE: method involves collecting blood received from donors into sterile triple containers containing blood preservative agent of CPDA-1. Low speed centrifuging is carried out not later than 2-5 h from the moment of taking blood samples at 2500-3500 x g during 15-20 min at 22-24 C. Blood is separated into blood plasma, erythrocyte mass and leukothrombotic layer Blood plasma received by applying the method has formed blood elements in the following quantities: erythrocytes - 2.0-4.0 108/l, leukocytes - 5.5-9.5 106/l, blood platelets - 3.5-5.5 109/l. Factor VIII activity is equal to 0.7-1.6 MU/ml. General protein contents is equal to 55-65 mg/ml. Hemoglobin concentration in erythrocyte mass is equal to 250-270 g/l, hematocrit index is equal to 76-80%, leukocyte quantity is equal to 5.4-6.4 109/l, blood platelets - 120-156 109/l. The plasma is frozen at -40- 50 C during 30-40 min and stored for 12-24 months. The erythrocyte mass is cooled at +2-4 C and stored until its being used to 35-42 days. EFFECT: improved product quality. 6 cl
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