| 摘要 |
<p>PROBLEM TO BE SOLVED: To provide a method for freeze-preserving cells directly in the form of minced tissue in serum-free vitrified liquid without a cell dispersion treatment with digestive enzymes and/or cell culture. SOLUTION: This method for freeze-preserving cells comprises the following procedure: a tissue collected with a biopsy needle from a living body or collected from corpse soon after death at a slaughterhouse or the like is minced with scissors into a pasty form, which is then thoroughly washed with a phosphate buffer solution; the resultant minced tissue is put into a serum-free vitrified liquid into a minced tissue suspension, which is then put into a freeze-preserving container stuck with an individual body identification label and sealed up; the resultant container is chilled on liquid nitrogen, put thereinto, and then preserved in the liquid nitrogen.</p> |
