POSITIVE SELECTION VECTOR FOR BACILLUS SP.

摘要

A positive selection vector is provided useful for the transformation and screening of Gram positive bacteria and particularly Bacillus sp. for the presence of foreign DNA. The vector comprises a mutant gene encoding a signa l peptide processing mutation. Expression of the mutant gene in a Bacillus hos t cell which lacks the ability to metabolize sucrose is lethal when cells are grown in the presence of sucrose. Foreign DNA may be inserted into the vecto r so as to inactivate the mutant gene thereby permitting the cells to grow in the presence of sucrose and allowing for facile selection of transformants.< /SDOAB>