SELECTION BY MIRROR IMAGE DISPLAY

摘要

Non-naturally occurring binders to cell surface carbohydrates and sugars are identified by a screening process that employs immobilized enantiomers of such cell surface carbohydrates and sugars. Preferred non-naturally occurring binders include D- peptides and L- nucleic acids and are resistance to enzymatic degradation and clearance. Single-chain Fab sequences that bind to sialic acid and KDO in nano-molar affinity were identified by this process. Exemplary screening procedures employed D-KDO, L-sialic acid and an L-sialo-disaccharide have been attached to a solid support for selection of high-affinity binders.