发明名称 |
DESIGN OF A POLYEPITOPIC CONSTRUCT FOR THE INDUCTION OF HLA-A2.1 RESTRICTED HIV 1 SPECIFIC CTL RESPONSES USING HHD MICE |
摘要 |
H-2 class I negative, HLA-A2.1 transgenic HHD mice were used for a comparative evaluation of the immunogenicity of HLA-A2.1 restricted human tumour-associated CTL epitopes and HIV 1-derived epitopes. A hierarchy was established among these epitopic peptides injected into mice in IFA which correlates globally with their capacity to bind and stabilize HLA-A2.1 molecules. A tyrosine substitution in position 1 of the HIV 1-derived epitopic peptides, which increases both their affinity for and their HLA-A2.1 molecule stabilizing capacity, was introduced in a significant proportion of them. DNA immunizations were performed using a construct comprising nucleic acids encoding the epitopes inserted into the pre-S2 segment of the hepatitis B middle glycoprotein. CTL responses against most of the inserted epitopes could be elicited simultaneously in a single animal.
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申请公布号 |
WO0127291(A9) |
申请公布日期 |
2002.09.06 |
申请号 |
WO2000EP09902 |
申请日期 |
2000.09.29 |
申请人 |
INSTITUT PASTEUR;FIRAT, HUESEYIN;LEMONNIER, FRANCOIS;LANGLADE-DEMOYEN, PIERRE |
发明人 |
FIRAT, HUESEYIN;LEMONNIER, FRANCOIS;LANGLADE-DEMOYEN, PIERRE |
分类号 |
A61K39/00;C07K14/02;C07K14/16;C12N15/62;(IPC1-7):C12N15/62;A61K39/21;A61K39/29 |
主分类号 |
A61K39/00 |
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