TWO CHAMBER CELL CULTURE VESSEL

摘要

A two-chamber perfusion bioreactor counteracts a significant amount of shear stress while allowing high nutrient transfer and removal or waste and/or valuable bio-products, e.g., protein, hormones, growth factors, and monoclonal antibodies without disturbing the cell culture. Cells and tissues, contained in the growth chamber, are suspended in the growth media by rotation of the vessel (2) and are separated by a porous membrane (114, 124) from the feeding chamber. Nutrients and gases are transferred across the membrane from the feeding chamber to the growth chamber. Even dispersion of nutrients along the membrane barrier is accomplished through a novel nutrient dispersion flow path. The flow path is created by flowing media out a center shaft (8) in the vessel and along the front of the nutrient dispersion disk (10). The fluid then flows around the back of the disk (10) and exits the outer center return shaft (30). The two-chamber perfusion device allows the attachment of different types of human cells to represent various tissues in the human body.