| 摘要 |
<p>The present invention provides a method of transfer of a gene of interest from a first vector to a product vector comprising contacting a first and second vector in vitro with a site-specific recombinase so as to generate a co-integrate vector comprising the components of the first and second vector, and introducing the co-ingetrate vector to a prokaryotic host cell so as to generate a product vector by rolling circle replication, comprising the gene of interest.</p> |
