发明名称 |
METHODS FOR IDENTIFYING LIGAND BINDING SITES IN A BIOMOLECULE |
摘要 |
Methods of identifying ligand binding sites in a target molecule such as a polynucleotide or polypeptide, as well as methods for determining whether a particular site in a target molecule is at or near the ligand binding site, are provided. Ligand binding affinities corresponding to both the target molecule and a modified version thereof are compared, preferably using mass spectral analysis. The modified molecules, or test molecules, incorporate a modification capable of modulating ligand binding affinity when located at o r near the ligand binding sites. Binding site information is derived from the known position of the modification.
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申请公布号 |
CA2439085(A1) |
申请公布日期 |
2002.09.06 |
申请号 |
CA20022439085 |
申请日期 |
2002.02.21 |
申请人 |
ISIS PHARMACEUTICALS, INC. |
发明人 |
SWAYZE, ERIC E.;GRIFFEY, RICHARD H.;HOFSTADLER, STEVEN A. |
分类号 |
C12Q1/68;G01N33/566;G01N33/68;(IPC1-7):C12Q1/68;C07H19/00;C07H21/00;C07H21/02;C07H21/04;G01N33/53;G01N33/567 |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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