发明名称 |
Enhanced expression of transgenes |
摘要 |
The present invention provides for improved methods of gene transfer, both in vitro and in vivo. By treating neoplastic cells with a DNA-damaging agent prior to transduction with a transgene, the expression of the transgene is improved up to about 3-fold over the expression seen in the absence of the DNA-damaging agent treatment. This effect is not dependent on the tumor cell type, the method of DNA transduction or type of DNA-damaging agent. The effect is most dramatic when the transduction is performed about 1-3 days following treatment with the DNA-damaging agent.
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申请公布号 |
US2002123477(A1) |
申请公布日期 |
2002.09.05 |
申请号 |
US20010922490 |
申请日期 |
2001.08.03 |
申请人 |
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM |
发明人 |
CRISTIANO RICHARD J.;NGUYEN DAO |
分类号 |
A61K38/00;A61K48/00;C07K14/47;C12N15/67;C12N15/85;C12N15/86;C12N15/87;(IPC1-7):A61K48/00;A61K38/17;A61K31/66;A61K31/407;A61K31/175;A61K31/28 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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