摘要 |
This invention relates to a gamma -cyclodextrin glucanotransferase having novel properties, to a process for the production of the gamma -cyclodextrin glucanotransferase which comprises culturing a strain belonging to the genus Brevibacterium capable of producing cyclodextrin glucanotransferase, thereby allowing the strain to produce the gamma -cyclodextrin glucanotransferase in a culture medium, and subsequently collecting the enzyme, to a process for the production of cyclodextrin which comprises allowing the cyclodextrin glucanotransferase to react with substrate dissolved in a solution, thereby effecting formation of gamma -cyclodextrin as a main product, and to a method for increasing gamma -cyclodextrin yield without accompanying yield increase of total cyclodextrin, which comprises adding ethyl alcohol to a reaction solution in which gamma -cyclodextrin and beta -cyclodextrin are formed from starch by the action of gamma -cyclodextrin glucanotransferase, thereby effecting repressed formation of beta -cyclodextrin and enhanced formation of gamma -cyclodextrin. Development of the process of this invention has rendered possible low cost production of gamma -cyclodextrin and therefore expands practical use of gamma -cyclodextrin to the field of food and the like. |