摘要 |
(R)-2,3-butanediol dehydrogenase useful for the production of ketones, alcohols, and particularly optically active vicinal diols. It has been found that <I>Pichia angusta</I> produces novel (R)-2,3-butanediol dehydrogenase that shows the high activity and high stereoselectivity. Further, polynucleotide encoding this (R)-2,3-butanediol dehydrogenase was cloned, and the nucleotide sequence thereof was determined. The expression of the glycerol dehydrogenase was carried out in heterologous microorganisms. The dehydrogenase produces (R)-acetoin using NAD as a coenzyme and (2R, 3R)-2,3-butanediol by reducing 2,3-butanedione using NADH as a coenzyme. |