| 摘要 |
1292434 Allergenic extracts MILES LABORATORIES Inc 8 Jan 1970 [24 Jan 1969] 1052/70 Heading A5B An extract of an active substance capable of giving an allergenic response and containing physiologically active principles is prepared by (1) extracting the active substance with an aqueous extracting fluid; (2) separating the aqueous extracting fluid containing the watersoluble active principles from the insoluble material therein; (3) extracting the insoluble material with an organic solvent for proteinaceous substances; (4) separating the organic solvent containing the organic-solvent-soluble active principles from the insoluble material therein; and (5) separating the active principles contained in the organic solvent from the organic solvent. The organic solvent may be dioxane, 2-propanol, dimethylsulphoxide, ethylenediamine, hydrazine, N-methyl-acetamide, piperidine, formamide, N, N-dimethyl formamide, N, N-dimethyl acetamide and preferably a heterocyclic tertiary amine such as pyridine, quinoline, lutidine, collidine or picoline; optionally in admixture with an aqueous phase such as aqueous sodium hydroxide. The active principles may be separated from the organic solvent by conventional procedures, preferably by dialysis or by addition of an aluminium compound (e.g. potassium aluminium sulphate) to the solution thereby insolubilizing the active principles. The water soluble active principles may be combined with the organic-solvent-soluble active principles for therapeutic and diagnostic use. The active substance extracted may be pollen, dust, epithelium, mould, insect material, seed, feathers, or complex chemicals such as antibiotics.
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