| 摘要 |
A device and method for molecular or microanalytical sensing that includes a sample chamber (3) containing a sensing device a first and second microchannel (14, 16) fluidly connected to the sample chamber and at least one pump (11) for pumping a fluid sample back and forth from the first microchannel through the sample chamber to the second microchannel. At least one of the microchannels or the sample chamber has a width that causes molecular mixing of the sample by laminar flow as the sample is pumped back and forth. The microchannels may have a width of, for example, from about 10 mu m to about 1 mm or from about 50 mu m to about 500 mu m. The sample chamber may have a volume of, for example, about 1 nl to about 10 nl or from about 10 nl to about 100 nl. The method is particularly useful for assessing the interaction between molecules in a solution with molecules immobilized on a surface or with the surface itself as in, for example, a biosensor. |
