| 摘要 |
<p>An assay for the determination of an analyte in an aqueous sample includes the steps of binding a first entity having an affinity for the analyte to a solid support. The first entity is bonded with the analyte to form a first complex. The first complex is reacted with a second entity to produce a second complex that is tagged with an enzyme. The second complex is combined with a substrate wherein a third complex is formed. An amplification reagent is added. The sample is irradiated with photonic energy, whereby the combination of the amplification reagent and the photonic energy provides catalysis for the further production of the third complex. The absorbance (OD) of the sample is then measured.</p> |
