ALLELE-SPECIFIC STRAND DISPLACEMENT AMPLIFICATION PRIMERS AND METHOD

摘要

Disclosed and described is a method for allele-specific strand displacement amplification of target nucleic acids. The primer set used to perform allele-specific strand displacement amplification preferably comprises a sense primer and a complementary anti-sense primer wherein one end of the anti-sense primer preferably has a sequence complementary to the sense sequence of a target nucleic acid sequence containing a specific allele or nucleic acid base. The specific allele may include a base that is considered "normal" sequence or it may include a point mutation. The sense primer may incorporate a biotin moiety at its 5' end to facilitate in one embodiment the capture of amplicons to specific sites on a bioelectronic microarray.