SUPERSECRETABLE PEPTIDES, PROCESSES FOR THEIR PRODUCTION, AND PARALLEL IMPROVEMENT OF THE SECRETED FORM OF ONE OR MORE OTHER POLYPEPTIDES

摘要

The invention relates to an expression cassette of the form Px-Sx-Bn-(ZR)-transport peptide-(Z1Z2)-protein(Y)-(Z1Z2)-protein (Ym)-T; wherein the expression cassette codes for a transport peptide linked via a sequence Z1Z2 to a second protein which in turn is linked via Z1Z2 to a protein Y1 which either corresponds to Y or can be different from Y and the transport peptide improves the rate of secretion and Y and/or Ym where Px is any promoter DNA sequence selected in such a way that optimal yields of the protein of interest become obtainable; Sx is any DNA which, accordingly, encodes any signal or leader sequence which allows optimal yields; Bn is 1-15 genetically encodable amino acids or a chemical bond; Z is the codon of an amino acid selected from the group comprising Lys and Arg; Z1 is the codon of an amino acid selected from the group comprising Lys and Arg; Z2 is the codon of an amino acid selected from the group comprising Lys and Arg; protein Ym is a DNA sequence encoding any protein which can be produced and secreted by yeast (m=1-5) or is a chemical bon (m=0); R is an argine codon; transport peptide is a DNA sequence encoding a peptide which can be transported efficiently and which can pass membranes; protein Y is a DNA sequence encoding any protein which can be produced and secreted by yeast and whose biological activity, when Ym is not a chemical bond, is not impaired by a basic dipeptide extension or allows degradation of the extension by carboxypeptidases; T is an untranslated DNA sequence advantageous to the expression, and to processes for the production and use thereof.