Arrays of flowable or fixed particle sets are used in microfluidic systems for performing assays and modifying hydrodynamic flow. Also provided are assays utilizing flowable or fixed particle sets within a microfluidic system, as well as kits, apparatus and integrated systems comprising arrays and array members.
The application particularly focusses on methods of performing a plurality of chemical reactions, for instance nucleic acid synthesis and sequencing reactions, in said microfluidic devices.