| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for producing a protein necessary for completely post-transitional modification having therapeutic, research and industrial values by a cell-free completely post-transltional modified protein synthesis not by a cell culture method. SOLUTION: This method for producing the completely post-transltional modified protein is characterized in that, in a method for producing a completely post-transltional modified protein, the method is passed through a coupled cell- free completely post-transltional modified protein synthesis comprising (a) a process for adding a DNA or RNA having genetic information on the objective protein to a cell extract, (b) a process for adding a nucleic acid to the cell extract and (c) a process for adding endoplasmic reticulum (ER)/Golgi apparatus or endoplasmic reticulum/Golgi apparatus/plasma membrane or post-transltional modified machinery such as other organelle besides them in an amount sufficient to stimulate the production of the completely post-transltional modified protein to the extract.
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