NOVEL ASSAY METHOD

摘要

It is intended to provide a novel assay method whereby accurate data can be obtained in assaying a test substance in a biological sample with the use of a reaction system enabling the formation of 6-phosphogluconic acid. It is noted that 6-phosphogluconic acid is the final product in a reaction system of assaying glucose by using a coenzyme and that 6PGDH (6-phosphogluconate dehydrogenase) in blood cells reacts using NADP as a coenzyme to thereby results in errors in the assay data. In assaying a test substance in a biological sample with the use of a reaction system enabling the formation of 6-phosphogluconic acid, the above object can be achieved by excluding 6-phosphogluconate from the reaction system.