Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen

摘要

The invention relates to an expression cassette of the form Px-Sx-Bn-(ZR)-Hir (AsmR)-protein (Y) - T, where Px is any promoter DNA sequence, selected in such a way that optimal yields of the protein of interest become achievable; Sx is any DNA encoding a signal sequence or leader sequence which allows optimal yields; Bn is 1-15 amino acid codons or a chemical bond; Z is the codon of a amino acid selected from the group comprising Lys and Arg; R is an Arg codon or a chemical bond; Asm is a chemical bond or m amino aid codons, where m= 1-10; Hir is a DNA sequence coding for hirudin or a hirudin derivative which is at least 40 % homologous to natural hirudin; protein Y is a DNA sequence encoding any protein which can be produced in and secreted by yeast; T is an untraslated DNA sequence which is advantageous to the expression.