| 摘要 |
The invention relates to a method for detecting and/or quantifying an analyte in a liquid, especially nucleic acids. Said method comprises the following steps: a) either first microparticles and a probe having a specific affinity for the analyte and for the first microparticles are prepared, or second microparticles are prepared, having a probe which is bound to the surface thereof, b) a first solution containing the analyte, the probe and the first microparticles is produced under conditions in which the probe binds itself to the analyte and to the first microparticles, or a first solution containing the analyte and the second microparticles is produced under conditions in which the analyte binds itself to the probe, c) the first or second microparticles are separated from the first solution, and d) the analyte is detected by means of an electrochemical method whereby the first or second microparticles are transferred into a second solution in order to detect the analyte. |
