发明名称 |
A METHOD OF PREPARING A VARIANT OF A LIPOLYTIC ENZYME |
摘要 |
<p>The present invention relates to a method of preparing a variant of a parent lipolytic enzyme, comprising (a) subjecting a DNA sequence encoding the parent lipolytic enzyme to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and (c) screening for host cells expressing a mutated lipolytic enzyme which has a decreased dependance to calcium and/or an improved tolerance towards a detergent or a detergent component as compared to the parent lipolytic enzyme.</p> |
申请公布号 |
EP0746618(B1) |
申请公布日期 |
2002.08.21 |
申请号 |
EP19950909666 |
申请日期 |
1995.02.22 |
申请人 |
NOVOZYMES A/S |
发明人 |
SVENDSEN, ALLAN;CLAUSEN, IB GROTH;OKKELS, JENS SIGURD;THELLERSEN, MARIANNE |
分类号 |
C12N15/09;C11D3/386;C11D7/42;C12N1/19;C12N1/21;C12N9/20;C12N15/55;C12R1/69;(IPC1-7):C12N15/55 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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