| 摘要 |
The present invention relates to a method for identifying compounds capable of modulating the function of a functional domain of human HIF-1alpha, said method comprising(i) contacting a candidate compound with a variant of human HIF-1alpha, said variant essentially lacking at least one functional domain of human HIF-1alpha, or having a mutation making at least one functional domain of human HIF-1alpha essentially inactive, said functional domain or domains being selected from the group consisting of(a) the PAS-B domain located in human HIF-1alpha between amino acids 178 and 390,(b) the C-terminal nuclear localization sequence (NLS) located in human HIF-1alpha essentially at amino acids 718 to 721, and(c) the transactivator/coactivator domain (N-TAD) located in human HIF-1alpha essentially between amino acids 531 to 584, and(d) the transactivator/coactivator domain (C-TAD) located in human HIF-1alpha essentially between amino acids 813 and 826, and(ii) determining the effect of the candidate compound on the said variant.
|
