发明名称 |
Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
摘要 |
Methods of providing shuffling libraries that include codon-varied oligonucleotide sequences are described. Codon-varied oligonucleotides are synthesized using trinucleotide or mononucleotide phosphoramidite sequences, and are derived from homologous or non-homologous nucleic acid sequences, or combinations of such sequences. Various methods of recombining codon-varied oligonucleotide sequences to expedite artificial evolution are also described. The present invention additionally relates to various integrated systems that are optionally used to automate these recombination methods.
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申请公布号 |
US6436675(B1) |
申请公布日期 |
2002.08.20 |
申请号 |
US19990408393 |
申请日期 |
1999.09.28 |
申请人 |
MAXYGEN, INC. |
发明人 |
WELCH MARK;NESS JON;GUSTAFSSON CLAES;STEMMER WILLEM P. C.;MINSHULL JEREMY |
分类号 |
C12N15/10;C12N15/11;G06F19/00;(IPC1-7):C12Q1/68;C12P19/34;C07H21/00 |
主分类号 |
C12N15/10 |
代理机构 |
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主权项 |
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地址 |
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