METHOD FOR IMMOBILIZING SUBSTANCES

摘要

A method for immobilizing substances by formation of plasma-polymerized films, after the arrangement of the substances to be immobilized on the surface of a support, and the immobilized substances according to the method are provided. The immobilized substances are retained with use of plasma-polymerized films, thereby damage to the immobilized substances is minimized, and the liberation of the substances after the immobilization will hardly occur. They can be further applied for indirect immobilization of biotinylated polynucleotides, because binding activities of the substances are kept on the surface. In addition, proteins having binding activities such as antibodies can be immobilized to be used for the detection of ligands. <IMAGE>