| 摘要 |
The Invention discloses a method for the analysis of the methylation of specific cytosine bases in genomic DNA samples, characterised by the fact that the following steps are implemented: (a) the genomic DNA is chemically treated in such a manner that cytosine is converted into uracil or a similar acting base regarding the base pairing behaviour in the DNA duplex, 5 methylcytosine however remains basically unmodified; (b) the chemically treated DNA is amplified using at least one oligonucleotide (type A) as primer in a polymerase reaction, whereby the two strands of the polymerase reaction product are manufactured in unequal quantities; (c) the amplificate is hybridised with one or more pairs of oligonucleotides (type B), which hybridise to the positions which are to be examined regarding their methylation status in the genomic DNA sample whereby one oligonucleotide of each pair hybridises preferentially in each case if in the genomic DNA sample the position was methylated, while the other oligonucleotide of the pair hybridises preferentially, if the position was unmethylated. Each oligonucleotide of a pair is labeled with a unique fluorescent label; (d) the fluorescence polarisation characteristics of the solution are measured, whereby for each fluorescent label used one determines the degree of polarisation.
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