METHOD FOR IMPROVING THE GENETIC STABILITY OF FOREIGN DNA SEQUENCE TO BE INSERTED INTO SINGLE-STRANDED RNA VIRUS RECOMBINANT VECTORS

摘要

PURPOSE: A method for improving the genetic stability of a foreign DNA sequence to be inserted into single-stranded RNA virus recombinant vectors is provided, thereby the genetic stability of the foreign DNA sequence within single-stranded RNA virus recombinant vectors can be significantly improved. CONSTITUTION: The method for improving the genetic stability of a foreign DNA sequence to be inserted into single-stranded RNA virus recombinant vectors comprises inducing mutation of the foreign DNA sequence to have uniform G/C ratio over the total foreign DNA sequence, in which the single-stranded RNA virus recombinant vector is selected from the group consisting of Yellow fever virus vector, Venezuelan equine encephalitis virus vector, Rubella virus vector, and Coxsackievirus vector; the uniform G/C ratio can be accomplished by increasing the amount of G/C in the foreign DNA sequence; and the G/C ratio of the foreign DNA sequence to the total foreign DNA sequence is 40% or more.