WATER-SOLUBLE, FLUORESCENT AND MOBILE PEPTIDE SUBSTRATES

摘要

The present invention provides non-radioactively labeled synthetic substrate s for enzymatic reactions which exhibit markedly improved solubility having th e general structure *F-R1-L1-R2-PHc1-PS-PHc2-(R3-L-R4-T)y. These substrates ma y be designed to carry a charge to allow electrophoretic separation of substrates and reaction products. The invention also provides enzymatic activity assays for protein kinases, phosphatases and proteases utilizing th e substrates of the invention, as well as methods of producing these substrate s. In addition, the invention also provides libraries of the substrates, and methods of utilizing these libraries to select optimal synthetic peptide enzyme substrates for high-throughput screening assays.