Vektorsystem zum Screening von Wirkstoffen

摘要

The invention relates to a vector system for use in screening active substances that influence translation in the cell. The inventive system is suitable for use in the pharmaceutical industry, in medicine and in molecular biology. The vector system consists of control elements of translation that substantially lie in the 5' position with respect to the detection cassette, but optionally also in the 3' position or within the detection cassette. The system further comprises a detection cassette that encodes two or more protein reporter constructs in different reading frames, said reporter constructs containing signal sequences for the release from the cell and various antigen sites by way of which reporter proteins can be immobilized and specifically detected.