发明名称 |
Recombinational cloning using engineered recombination sites |
摘要 |
<p>Compositions and kits are provided for recombinational cloning. One composition comprises at least one isolated recombination protein and at least one isolated nucleic acid molecule comprising at least a first recombination site containing at least one mutation, wherein said mutation removes one or more stop codons from said recombination site. Another composition comprises at least one isolated recombination protein and at least one nucleic acid molecule comprising at least a first nucleic acid sequence selected from the group consisting of SEQ ID NOs:1-16, a complementary DNA sequence thereto, and an RNA sequence corresponding thereto. A further composition comprises at least one isolated recombination protein and at least one isolated nucleic acid molecule comprising at least a first recombination site containing at least one mutation, wherein said mutation avoids hairpin formation. Other compositions are as further defined in the claims.</p> |
申请公布号 |
EP1229113(A2) |
申请公布日期 |
2002.08.07 |
申请号 |
EP20020001135 |
申请日期 |
1996.06.07 |
申请人 |
INVITROGEN CORPORATION |
发明人 |
HARTLEY, JAMES L.;BRASCH, MICHAEL A. |
分类号 |
C12N15/09;A61K48/00;C07K19/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/00;C12N15/10;C12N15/64;C12N15/66;(IPC1-7):C12N15/10;C12N15/63;C12N15/85 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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