发明名称 |
METHOD FOR PREPARING CELL FRACTION CONTAINING HEMANGIOBLASTS |
摘要 |
<p>Mouse PCLP1 was identified by expression cloning with the use of a monoclonal antibody against a surface antigen of a cell line derived from mouse AGM. By fractionating PCLP1-positive/CD45-negative cells and culturing them in vitro, it was clarified that these cells differentiate into endothelial-like cells, angioblast-like cells, and hematopoietic cells. By transferring the PCLP1-positive/CD45-negative cells into a mouse defective in the hematopoietic function, the hematopoietic system was reconstructed over a long period of time. These facts indicate that the PCLP1-positive/CD45-negative cells contain mammalian hemangioblasts capable of expressing the activity as long--term repopulating hematopoietic stem cells (LTR-HSC). The present invention provides a method for preparing a cell fraction containing hemangioblasts, the cell fraction prepared by the method, and use of this cell fraction. <IMAGE></p> |
申请公布号 |
EP1229116(A1) |
申请公布日期 |
2002.08.07 |
申请号 |
EP20000971829 |
申请日期 |
2000.11.07 |
申请人 |
CENTER FOR ADVANCED SCIENCE AND TECHNOLOGY INCUBATION, LTD. |
发明人 |
MIYAJIMA, ATSUSHI;HARA, TAKAHIKO |
分类号 |
A01K67/027;C07K16/18;C07K16/28;C12N5/074;C12N5/0789;C12N15/12;C12P21/08;(IPC1-7):C12N15/12;C12Q1/08;C12N5/00;C07K14/47 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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