| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for recombinant gene formation, with which a recombinant gene can be formed in the body of a microorganism, the function of the recombinant gene is evaluated in the body of microorganism as it is and an objective gene can be directly searched. SOLUTION: This method for recombinant gene formation is a method for recombining two or more kinds of genes having homology in the same body of microorganism in which a first plasmid vector obtained by connecting one gene to a specific base sequence derived from Bacillus sp.KSM-KP43, a base sequence in which one or more bases are deleted or substituted or added in the base sequence or a base sequence encoding a specified amino acid sequence different from that of the base sequence derived from Bacillus sp.KSM-KP43 or an amino acid sequence in which one or more amino acids are deleted, substituted or added in the amino acid sequence and the other plasmid vector obtained by connecting the other gene to a replication initiation domain different from the first plasmid vector are transferred to a microorganism and homologous recombination is carried out, between the genes. |
