Method of sample introduction for supercritical fluid chromatography systems

摘要

An improved method and apparatus for safely introducing sample into a flow stream of a supercritical fluid chromatography system is described. The sample injection method improves safety of injection by eliminating the risk of injecting sample into a high-pressure system containing liquids and gasses at or near supercritical ranges. The method improves the efficiency and significantly shortens the time of the injection process by using a check valve placed between a fill port on an injection valve and sample port where sample is injected by a syringe. After sample injection from a sample loop into a high-pressure flow stream, an injection valve is reset to receive a subsequent sample. High-pressure flow stream mixtures become trapped within the sample loop when the valve is reset. The check valve prevents the mixtures from expanding into the sample port and blowing out a syringe and flow stream mixtures from the fill lines. The trapped flow stream mixtures are directed to a waste port where they are safely contained. The technique can be applied equally as effective to preparatory SFC as well as to analytical SFC systems.