| 摘要 |
A sample preparation apparatus for DNA analysis comprises a holder for separating specific primers on the basis of size, color, weight, dimension, or degree of magnetization, the specific primers having base sequences complementary to a plurality of DNA fragments to be amplified via PCR, and the specific primers being capable of binding respectively to the DNA fragments; and a reaction-solution-holding plate having a concavity which accommodates one edge of the holder and a PCR solution containing a common primer capable of hybridizing with the base sequence of an oligonucleotide introduced into the 5'-end of each of the DNA fragments, and the DNA fragments. The PCR amplification of the DNA fragments is carried out by using the specific primers (immobilized on the surfaces of a plurality of mutually separable supports with respect to each DNA fragments) and the common primer (a mobile primer common to all DNA fragments) to produce PCR amplification products inside the corresponding portions of the holder. The DNA fragments are derived from a plurality of DNAs to be amplified by PCR under the same conditions at the same time to avoid undesired mutual interference among the primers, and the PCR products can be separated and recovered for each of the DNA fragments. The sample preparation method for DNA analysis comprises the relevant amplifying, separating and recovering steps as described above.
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