AMMONIUM SULFATE FOR NEUTRALIZATION OF INHIBITORY EFFECTS

摘要

Ribonucleic acid (RNA) is a substance synthesized biologically and synthetically. RNA serves many functions as information molecules, reaction substrate, reaction catalyst, recognition element, structural element etc. For most analysis methods and functions concerning RNA itself or RNA as an analysis tool the purity of RNA is important. That means that e.g., other molecules which bind the RNA are able to inhibit the analysis/function of/using the RNA molecule or destroy the structure of the RNA molecule. For this reason it is important to eliminate the inhibitory or destroying function of molecules binding to the RNA. Moreover stable secondary structures of RNA often results in reduced function and analysis quality. Secondary structure depends on the environment of the RNA molecule. The present invention features the addition of ammonium sulfate (NH>4<)>24< to RNA in solution to eliminate the inhibitory or destroying effect of molecules binding to the RNA to be taken as information molecule, reaction substrate, reaction catalyst, recognition element, structural element etc. Furthermore (NH>4<)>24< solves secondary structures of RNA to make RNA more accessible to reactions and analysis.