发明名称 |
Transgenic animals |
摘要 |
The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
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申请公布号 |
US2002104109(A1) |
申请公布日期 |
2002.08.01 |
申请号 |
US20010849566 |
申请日期 |
2001.05.04 |
申请人 |
BREMEL ROBERT D.;BLECK GREGORY T. |
发明人 |
BREMEL ROBERT D.;BLECK GREGORY T. |
分类号 |
A01K67/027;C07K14/02;C12N5/02;C12N15/85;C12N15/867;(IPC1-7):A01K67/027 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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