| 摘要 |
<p>A method is provided for differentiating embryonic stem cells to endocrine cells. The method includes generating embryoid bodies from a culture of undifferentiated embryonic stem cells, selecting endocrine precursor cells, expanding the endocrine precursor cells by culturing endocrine cells in an expansion medium that comprises a growth factor, and differentiating the expanded endocrine precursor cells in a differentiation media to differentiated endocrine cells produced by this method are also provided. Artificial islets are disclosed, as well as method for using the pancreatic endocrine cells and the artificial islets.</p> |
