CHOLESTEROL ESTERASES WITH VARIABLE SUBSTRATE SPECIFICITY

摘要

2064202 9118987 PCTABS00008 Cholesterol esterases with variable substrate specificity are made by cloning of a cholesterol esterase gene whose active centre contains the sequence -Gly-His-Ser-X-Gly, where X stands for an amino acid, in a vector, transformation of a microorganism with this vector and expression of the cholesterol esterase gene. The amino acid X of the active centre is interchanged with another amino acid by mutagenesis.