一种人的血清抑制基因

摘要

本发明涉及一种人的血清抑制基因,属生物学技术领域。本发明采用计算机与网络资料查询,并按现有技术进行计算机克隆,获得一个全长1.478Kb的序列,在人类基因组中第12号染色体短臂24.1位点(12q24.1)找到了全部序列,并通过PCR扩增获,得到了该序列全部编码序列。人的血清抑制基因(Si－1)具有能够控制G1/S过度点的作用,对了解细胞周期控制的机制有重要的参考价值,具有可应用于肿瘤诊断与治疗等优点。

主权项

一种人的血清抑制基因，其特征在于该血清抑制基因全长1.478Kb，蛋白质编码框为365个氨基酸残基；其氨基酸序列和核苷酸序列分别如下：MLWKLLLRSQSCRLCSFRKMRSPPKYRPFLACFTYTTDKQSSKENTRTVEKLYKCSVDIRKIRRLKGWVLLEDETYVEEIANILQELGADETAVASILERCPEAIVCSPTAVNTQRKLWQLVCKNEEELIKLIEQFPESFFTIKDQENQKLNVQFFQELGLKNVVISRLLTAAPNVFHNPVEKNKQMVRILQESYLDVGGSEANMKVWLLKLLSQNPFILLNSPTAIKETLEFLQEQGFTSFEILQLLSKLKGFLFQLCPRSIQNSISFSKNAFKCTDHDLKQLVLKCPALLYYSVPVLEERMQGLLREGISIAQIRETPMVLELTPQIVQYRIRKLNSSGYRIKDGHLANLNGSKKEFEANFGKIQAKKVRP 1 ggtgctgaga actgtcggga gcttcttgag accgaggacc gaaatcccgg ctccaggcct 61 cggggactgc ggactgtggg gaggctggcc ggagagagag ggaaggacgg ggcctggccc 121 ccgggactcc ctgtgccttg cttggagctg acgccgacgg ggaactgaca agatcacatt 181 ttgagaagaa gttggaaaga atcccaagtg gatgaactga atatctggat gaggacaaga 241 tctgtgggga gagactgtaa gatagaatga gtccatttaa gtcccaggac ggtggaaact 301 agctagtaga ttgcagccat gttgtggaag ctgctgctga gatcccagtc ctgcaggctg 361 tgttctttca gaaagatgcg atcacctcca aaatacagac ctttcttagc atgcttcacc 421 tatacaactg ataaacagtc gagcaaagaa aatacaagaa cagtggaaaa gctctataaa 481 tgttcagttg acattaggaa aattcgtaga ttaaaaggat gggtactttt agaggatgaa 541 acctatgttg aagaaattgc gaatatttta caagaactag gtgccgatga gactgctgta 601 gccagtattt tggaacgctg cccggaagca attgtctgta gtccaaccgc tgttaacacc 661 cagagaaaac tctggcagtt ggtctgcaaa aatgaggaag agttaatcaa gttaatagag 721 cagtttccag aatctttctt tactattaaa gaccaagaga accagaagct gaatgttcag 781 ttctttcaag agttgggact aaaaaatgtg gtcattagca gacttttgac agctgcacct 841 aatgtttttc ataatcctgt tgagaagaat aagcaaatgg taagaattct ccaagagagt 901 tatctagatg taggtggctc tgaggccaac atgaaagttt ggctactaaa attgttaagc 961 caaaacccat ttattttgtt aaattctccc acagctataa aggaaacact agaatttctc 1021 caggagcaag gtttcaccag ctttgaaatt ctccagcttc tatccaaact caaaggattt 1081 ctttttcaac tttgcccaag aagtatacag aatagtattt ccttctctaa aaatgctttt 1141 aaatgcacag atcatgacct gaagcaatta gttttgaaat gtcctgccct tttatattat 1201 tctgttccag ttttagaaga gagaatgcaa ggattattga gagaaggaat ttccatagct 1261 cagataagag agacgccaat ggttcttgaa ttaacaccac agatagtaca gtacaggata 1321 aggaaactga attcctcagg ctacagaata aaggatggac atctagcaaa tctaaatgga 1381 tcaaaaaaag agtttgaagc taattttggc aaaattcagg ccaaaaaagt aaggccatta 1441 tttaaccctg tggcaccatt aaatgttgaa gaatgaca