发明名称 |
LIGAND-AMINODEXTRAN-MARKER CONJUGATES AND USES THEREOF |
摘要 |
<p>Ligand-aminodextran-(phycobiliprotein or tandem dye) conjugates useful for detection of a desired target biological material by providing an enhanced fluorescent signal are described. Also described is a method for a single-measurement quantification of multiple populations of cells based upon the labeling of different pairs of cell populations, each pair containing mutually exclusive cell receptors which are expressed at substantially similar receptor densities with labeled ligands for each receptor. One cell population is labeled with a ligand capable of binding to a first cell surface receptor which ligand is directly conjugated to a fluorescent phycobiliprotein or tandem dye; and a second cell population is labeled with a ligand capable of binding to a second cell surface receptor, which ligand is cross-linked to an aminodextran to a fluorescent phycobiliprotein or tandem dye.</p> |
申请公布号 |
EP0981747(B1) |
申请公布日期 |
2002.07.31 |
申请号 |
EP19980922261 |
申请日期 |
1998.05.14 |
申请人 |
COULTER INTERNATIONAL CORP. |
发明人 |
SIIMAN, OLAVI;BURSHTEYN, ALEXANDER;MYLVAGANAM, RAVINDRA;RAYNOR, ROBERT;ROTH, PATRICIA;SMITH, CECILIA;WILKINSON, JULIE |
分类号 |
G01N33/548;G01N15/14;G01N33/483;G01N33/533;G01N33/543;G01N33/566;G01N33/569;(IPC1-7):G01N33/533 |
主分类号 |
G01N33/548 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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