发明名称 |
METHODS OF DETECTING POLY(ADP-RIBOSE) POLYMERASE ENZYMATIC ACTIVITY |
摘要 |
The invention provides methods for assaying poly(ADP-ribose) polymerase (PARP) activity and methods for the identification of modulators of PARP activity. These methods comprise contacting immobilized PARP with N AD under conditions that allow PARP auto-ribosylation, contacting the auto- ribosylated PARP with a detectable marker to form a complex and then indirectly or directly measuring the amount of detectable marker bound to the auto- ribosylated PARP, this amount being indicative of PARP activity.
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申请公布号 |
CA2369408(A1) |
申请公布日期 |
2002.07.30 |
申请号 |
CA20022369408 |
申请日期 |
2002.01.28 |
申请人 |
PFIZER PRODUCTS INC., |
发明人 |
MARALA, RAVI BALAYA;BROWN, JANICE ANN |
分类号 |
G01N33/50;C12Q1/25;C12Q1/42;C12Q1/48;G01N33/15;G01N33/566;G01N33/573;G01N33/58;G01N33/68;(IPC1-7):C12Q1/48;G01N33/533 |
主分类号 |
G01N33/50 |
代理机构 |
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