DNA ENCODING TRANSMEMBRANE DOMAIN-LACKING TYPE N- ACETYLGLUCOSAMINE DEACETYLATION ENZYME

摘要

PROBLEM TO BE SOLVED: To efficiently obtain an N-acetylglucosamine deacetylation enzyme. SOLUTION: This DNA encoding the partial polypeptide of the polypeptide of an N-acetylglucosamine N-deacetylation-N-sulfate group transfer enzyme is characterized in that the partial polypeptide obtained by inserting the DNA into an expression vector and then expressing the partial polypeptide does not have the N-terminal domain and C-terminal domain of the polypeptide of the N-acetylglucosamine N-deacetylation-N-sulfate group transfer enzyme in such an extent as capable of having an action for deacetylating the N- acetylglucosamine residue of a sugar chain having a heparin skeleton. A host cell is transformed with an expression vector containing the DNA, and the obtained transformant is cultured.