A METHOD FOR CREATING ENDOTHELIAL CELL DYSFUNCTION IN CELL CULTURE

摘要

The proposed invention relates to a method for creating cell culture conditions which causes human endothelial cells in culture when exposed to a cytokine, preferably, TNF.alpha. for periods of longer than 5 days (chronic exposure) to become unresponsive to growth factors and other substances in serum. The cell culture assay system allows for screening for novel molecules that may corre ct endothelial dysfunction and will help identify genes that could be used to prevent or correct endothelial dysfunction with gene therapy.